MOLECULAR CHARACTERIZATION OF WAX APPLE CULTIVAR (Syzygium samarangense (Blume) Merr. & L.M. Perry) UTILIZING psbA-trnH INTERGENIC SPACER

  • Tiara Dwi Meilina
  • Syifara Chika
  • Muhammad Rifqi Hariri Research Center for Biosystematics and Evolution, National Research and Innovation Agency
  • Asri Febriana Department of Biology, Faculty of Science and Technology, Universitas Islam Negeri Walisongo Semarang
  • Arnia Sari Mukaromah UIN Walisongo Semarang

Abstract

Wax apple (Syzygium samarangense (Blume) Merr. & L.M. Perry) is an agricultural comodities located in Demak Regency, Central Java. Authentication with DNA Barcoding is important to prove the authenticity of DNA-based species because it is difficult to distinguish morphologically, one of which uses the DNA Barcode psbA-trnH. This study aims to analyze the characteristics of the psbA-trnH intergenic spacer sequence in wax apple cultivar (S. samarangense) from Demak regency, analyze the genetic relationship between wax apple cultivar (S. samarangense) from Demak regency with data contained in GenBank based on the DNA barcode psbA-trnH, and determine the results of DNA Barcoding of psbA-trnH chloroplast DNA sequences can be used in wax apple authentication. Results of the study showed that the psbA-trnH sequence in wax apple (S. samarangense) of the Madu Thailand and Madu Deli Hijau cultivars had 535 and 492 bp, respectively. The composition of deoxyadenylic acid (A) and deoxythymidylic acid (T) in both cultivars is more than the composition of deoxycytidylic acid (C) and deoxyguanylic (G). The genetic distance between the wax apple (S. Samarangse) cultivars of Madu Thailand and Madu Deli Hijau has a close relationship with a matrix value 0.000. DNA Barcode psbA-trnH cannot discriminate the wax apple cultivar (S. samarangense) from Demak regency because its ability to separate wax apple cultivars is very low.

Keywords: Authentication, DNA Barcoding, psbA-trnH intergenic spacer, Syzygium samarangense, Wax Apple

 

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Published
2024-07-02
How to Cite
MeilinaT. D., ChikaS., HaririM. R., FebrianaA., & MukaromahA. S. (2024). MOLECULAR CHARACTERIZATION OF WAX APPLE CULTIVAR (Syzygium samarangense (Blume) Merr. & L.M. Perry) UTILIZING psbA-trnH INTERGENIC SPACER. Jurnal Ilmu Pertanian Indonesia, (00). Retrieved from https://jurnalpenyuluhan.ipb.ac.id/index.php/JIPI/article/view/50804
Issue
Vol. 0 No. 00 (2024): inpress
Section
Articles
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